Multiple genes encode the human Na+,K+-ATPase catalytic subunit.

M M Shull; J B Lingrel

doi:10.1073/pnas.84.12.4039

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Multiple genes encode the human Na+,K+-ATPase catalytic subunit.

M M Shull·J B Lingrel

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Abstract

A human genomic library was constructed and screened with hybridization probes derived from sheep and rat cDNAs encoding the alpha and alpha(+) isoforms, respectively, of the Na+,K+-ATPase catalytic subunit. Genomic sequences spanning 150 kilobases were isolated. Four genes, designated alpha A, alpha B, alpha C, and alpha D, each 20-25 kilobases in length, were identified by restriction mapping, Southern blot hybridization analysis, and limited DNA sequencing. We present evidence that two of these genes, alpha A and alpha B, encode the alpha and alpha(+) isoforms, respectively. The other genes, alpha C and alpha D, one of which is physically linked to the alpha(+) gene, exhibit nucleotide and amino acid homology to Na+,K+-ATPase catalytic subunit cDNA sequences but do not correspond to any previously identified isoforms.

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Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA DNA Restriction Enzymes Genes Humans Kidney Leukocytes Macromolecular Substances Nucleic Acid Hybridization Sheep Sodium-Potassium-Exchanging ATPase Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M M Shull, J B Lingrel. 1987. Multiple genes encode the human Na+,K+-ATPase catalytic subunit.. https://doi.org/10.1073/pnas.84.12.4039

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