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PubMed · 3090943

Selective decrease of 15-hydroxyeicosatetraenoic acid (15-HETE) formation in uninvolved psoriatic dermis.

K Kragballe·E A Duell·J J Voorhees
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Abstract

Epidermis of psoriatic skin lesions is characterized by elevated 5-lipoxygenase and 12-lipoxygenase products. 15-hydroxyeicosatetraenoic acid (15-HETE), the predominant lipoxygenase product in normal dermis, has the potential to inhibit 5-lipoxygenase and 12-lipoxygenase. The purpose of the present study was to determine the capacity of homogenized dermis from uninvolved psoriatic skin to form 15-HETE in vitro. Extracted lipids were separated by reversed-phase high-performance liquid chromatography. Each chromatographic peak was identified by its coelution with authentic standards, by ultraviolet spectrometry, and by radioimmunoassay. Dermis from uninvolved psoriatic skin generated on average 48% less 15-HETE than normal dermis (P less than .01). In contrast, the formation of 12-hydroxyeicosatetraenoic acid was increased by 56% in psoriatic dermis (P less than .01). Prostaglandin E2 formation was similar in normal and psoriatic dermis. Since 15-HETE can inhibit the synthesis of 5-lipoxygenase and 12-lipoxygenase products that possess inflammatory and proliferative capacities, a defective 15-HETE generation in dermis may be of importance for the development of psoriasis.

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12-Hydroxy-5,8,10,14-eicosatetraenoic AcidArachidonic AcidArachidonic AcidsChromatography, High Pressure LiquidDinoprostoneHumansHydroxyeicosatetraenoic AcidsIn Vitro TechniquesProstaglandins EPsoriasisRadioimmunoassaySkinJournal ArticleResearch Support, Non-U.S. Gov't

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K Kragballe, E A Duell, J J Voorhees. 1986. Selective decrease of 15-hydroxyeicosatetraenoic acid (15-HETE) formation in uninvolved psoriatic dermis.. https://pubmed.ncbi.nlm.nih.gov/3090943/

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