A simple microassay for interleukin-2 activity.

I Ando; P C Beverley

doi:10.1016/0022-1759(87)90377-2

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A simple microassay for interleukin-2 activity.

I Ando·P C Beverley

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Abstract

A simple in vitro assay is described to quantitate human interleukin-2 (IL-2). It is based on the spontaneous' activation of human peripheral T cells in tissue culture and the subsequent expression of the IL-2 receptor. The results obtained with this assay agree with those of assays which utilise IL-2 dependent T cell clones. The method is highly sensitive, reproducible and easy to perform.

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Antigens, Differentiation, T-Lymphocyte Antigens, Surface Cells, Cultured Growth Substances Humans Interleukin-2 Lymphocyte Activation Microchemistry Monocytes Phenotype Phytohemagglutinins Receptors, Immunologic Receptors, Interleukin-2 Recombinant Proteins T-Lymphocytes Journal Article

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I Ando, P C Beverley. 1987-01-26. A simple microassay for interleukin-2 activity.. https://doi.org/10.1016/0022-1759(87)90377-2

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