[Anti-CD3 monoclonal antibodies. Characterization and function].

D Bourel; N Genetet; G Merdrignac; B Genetet

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PubMed · 3125512

[Anti-CD3 monoclonal antibodies. Characterization and function].

D Bourel·N Genetet·G Merdrignac·B Genetet

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Abstract

Four anti-T lymphocyte monoclonal antibodies were produced by the hybridoma technique. Percentages of miscellaneous labelled cell suspensions, as profiles of fluorescent histograms, showed a reactivity of these 4 reagents quite comparable to that observed with anti-CD3 monoclonal antibodies. Labelling summation studies, cell-sorting studies and blocking experiments gave similar results as gave other anti-CD3 reagents. Biochemical study and functional tests: modulation effects and mitogenic properties, argued for the belonging of these 4 antibodies to the CD3 cluster. The actual data concerning the CD3 molecule are briefly resumed to underline the interest of such antibodies in the understanding of T-lymphocyte activation mechanisms during the immune response.

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Animals Antibodies, Monoclonal Antibody Specificity Antigens, Differentiation, T-Lymphocyte Antigens, Surface Flow Cytometry Fluorescein-5-isothiocyanate Fluoresceins Fluorescent Dyes Hybridomas Leukemia, Lymphoid Lymphocyte Activation Mice Mice, Inbred BALB C Mitosis T-Lymphocytes Thiocyanates English Abstract Journal Article

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D Bourel, N Genetet, G Merdrignac, B Genetet. 1987. [Anti-CD3 monoclonal antibodies. Characterization and function].. https://pubmed.ncbi.nlm.nih.gov/3125512/

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