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Isolation of type 5 capsular polysaccharide from Staphylococcus aureus.

J M Fournier·K Hannon·M Moreau·W W Karakawa·W F Vann
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Abstract

Clinical surveys have indicated the predominance of type 5 and type 8 capsular polysaccharides among clinical isolates of Staphylococcus aureus. The type 5 capsular polysaccharide was extracted from three clinical isolates of S. aureus grown on solid media, and purified by ion exchange chromatography and gel filtration. Chemical analysis showed that the type 5 capsular polysaccharide is composed of N-acetylfucosamine and N-acetylhexosaminuronic acid. Immunodiffusion and 13C nuclear magnetic resonance experiments showed that the type 5 capsular polysaccharide is both immunologically and chemically distinct from the type 8 capsular polysaccharide and the teichoic acid of S. aureus.

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Bacterial CapsulesChromatography, GasChromatography, GelChromatography, Ion ExchangeElectrophoresis, PaperHumansImmunodiffusionMagnetic Resonance SpectroscopyPolysaccharides, BacterialStaphylococcus aureusJournal Article

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J M Fournier, K Hannon, M Moreau, W W Karakawa, W F Vann. Isolation of type 5 capsular polysaccharide from Staphylococcus aureus.. https://doi.org/10.1016/0769-2609(87)90041-x

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