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Synchronization of MEL cell commitment with cordycepin.

R Levenson·J Kernen·D Housman
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Abstract

The response of differentiating MEL cells to the nucleotide analogue cordycepin reveals a previously unrecognized aspect of the molecular events which cause commitment of these cells to terminal erythroid differentiation. Cordycepin rapidly inhibits commitment of DMSO-treated MEL cells in a dose range which does not cause cytotoxicity. Reversal of cordycepin treatment in the presence of inducer leads to a rapid and synchronous commitment of a significant proportion of cells in the culture. These results suggest that MEL cells can be blocked just prior to the point of commitment by cordycepin treatment.

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AnimalsCell DifferentiationCell DivisionCell LineDeoxyadenosinesDimethyl SulfoxideErythropoiesisLeukemia, Erythroblastic, AcuteMiceRNAJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R Levenson, J Kernen, D Housman. 1979. Synchronization of MEL cell commitment with cordycepin.. https://doi.org/10.1016/0092-8674(79)90220-4

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