[Technics for B-cell determination in lymphocytes].

Abstract

The conception of the subdivision of lymphocytes into T- (dependent on thymus) and B- (dependent on bone marrow or bursa) cells which was transferred from animal experiments on mice to man has already clinical significance despite some deficiencies. For immunoproliferative clinical pictures, immune defects and autoimmune diseases a determination of the B- and T-lymphocytes in the peripheral blood is important. As methods for the determination of T-lymphocytes the rosette-test with erythrocytes of sheep (E-rosettes) as well as the immunofluorescence labelling of anti-T-lymphocyte globulin are used. For the demonstration of B-lymphocytes to-day the immunofluorescence microscopy, the autoradiography, the immune enzyme technique and the rosette tests for the determination of the Fc-receptor (EA-rosette) and the complement receptor (EAC-rosette), respectively, are used. Possibilities of differentiation between the two populations of lymphocytes are furthermore obtained by the grid electron microscopy and the important functional tests of the transformation of lymphocytes by means of unspecific and specific stimulants. In own investigations by means of the direct and indirect immunofluorescence and immune enzyme technique of the peripheral blood and from lymphatic nodes were tested. In normal persons a large proportion of IgM-receptors on the lymphocyte membrane was established. Patients with chronic lymphadenosis had a high number of B-lymphocytes, which clearly decreased under cytostatic therapy. In plasmocytoma, Waldenström's disease and primary immune defects the connections between the dominating classes of immunoglobulins on the membrane and in the serum. Investigations of the different populations of lymphocytes in there are peripheral blood and in the lymphatic nodes are suited to develop new conceptions on pathogenetic intergrades, therapeutic influences and variants of the course of immunoproliferative and immunodefective clinical pictures.