Three-dimensional computer reconstructions from serial sections of cell nuclei.

G Geraud; A Soyer; Y Epelboin

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PubMed · 3390621

Three-dimensional computer reconstructions from serial sections of cell nuclei.

G Geraud·A Soyer·Y Epelboin

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Abstract

The analysis of ultrathin serial sections as 3-dimensional (3D) information requires interpretation and display of a large amount of data. We suggest a simple way to solve this problem; it permits presentation of a series of sections as a 3D color image of good quality. It involves a picture system with specialized hardware and software written for this purpose. 3D images of cellular organelles have been drawn either by manually defining the contour of the objects or by thresholding of the volumes in the structures. These 2 methods allow rapid drawing of the image on the screen. It is possible to determine the position, shape and size of 3D structures. This interactive system allows the user to choose between several options: colors, removal of parts of the object, and cutout.

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Animals Cell Nucleus Computer Simulation Computers Cricetinae Image Processing, Computer-Assisted Microscopy, Electron Journal Article

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G Geraud, A Soyer, Y Epelboin. 1988. Three-dimensional computer reconstructions from serial sections of cell nuclei.. https://pubmed.ncbi.nlm.nih.gov/3390621/

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