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PubMed · 3426640

[Modification of nucleic acids in stabilized complementary complexes. II. Effectiveness and direction of alkylation of dodecadeoxyribonucleotide d(pA-A-C-C-T-G-T-T-T-G-G-C) with 4-(N-2-chloroethyl-N-methylamino)benzylidene derivative of the complementary heptanucleotide d(pC-C-A-A-A-C)-A containing a N-(2-hydroxyethyl)phenazinium residue at the 5'-end].

V F Zarytova·I V Kutiavin·M A Podyminogin·V N Sil'nikov·G V Shishkin
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Abstract

Modification of dodecadeoxyribonucleotide d(pA-A-C-C-T-G-T-T-T-G-G-C) (I) with a heptanucleotide 4-(N-2-chloroethyl-N-methylamino)benzylidene (RCI) derivative d(pC-C-A-A-A-C) ARCI (II) and with similar reagent (III) bearing an additional 5'-terminal N-(2-hydroxyethyl)phenazinium residue (Phn) has been unvestigated. Both reagents (II) and (III) alkylated dodecanucleotide (I) mainly at the 5'-terminal phosphate, Phn residue not affecting specificity of the alkylation. Stabilization of the complementary complex target oligonucleotide-oligonucleotide derivative by the Phn group resulted in substantial increase of efficiency and rate of the intracomplex alkylation of the dodecanucleotide.

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AlkylationBenzylidene CompoundsChemical PhenomenaChemistryOligodeoxyribonucleotidesPhenazinesEnglish AbstractJournal Article

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V F Zarytova, I V Kutiavin, M A Podyminogin, V N Sil'nikov, G V Shishkin. 1987. [Modification of nucleic acids in stabilized complementary complexes. II. Effectiveness and direction of alkylation of dodecadeoxyribonucleotide d(pA-A-C-C-T-G-T-T-T-G-G-C) with 4-(N-2-chloroethyl-N-methylamino)benzylidene derivative of the complementary heptanucleotide d(pC-C-A-A-A-C)-A containing a N-(2-hydroxyethyl)phenazinium residue at the 5'-end].. https://pubmed.ncbi.nlm.nih.gov/3426640/

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