Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins.

S Hase; S Natsuka; H Oku; T Ikenaka

doi:10.1016/0003-2697(87)90171-0

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Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins.

S Hase·S Natsuka·H Oku·T Ikenaka

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Abstract

A mixture of the pyridylamino (PA) derivatives of 12 oligomannose-type sugar chains was fractionated into five fractions (mannose5N-acetylglucosamine2-PA approximately mannose9N-acetylglucosamine2-PA) by size-fractionation HPLC with a MicroPak AX-5 column. Each fraction thus obtained was then analyzed by reversed-phase HPLC with a Cosmosil 5C18-P column. In this way, the 12 PA-oligomannose-type sugar chains were completely separated from each other. The method was used to identify the structures of oligomannose-type sugar chains of human C3, the third component of human complement.

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Carbohydrate Sequence Chromatography, High Pressure Liquid Complement C3 Glycoproteins Glycosides Humans Mannosides Molecular Sequence Data Oligosaccharides Journal Article Research Support, Non-U.S. Gov't

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S Hase, S Natsuka, H Oku, T Ikenaka. 1987. Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins.. https://doi.org/10.1016/0003-2697(87)90171-0

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