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PubMed · 3477467

Differentiation-inducing factor for a human leukemic cell line produced by colony-stimulating factor producing human lung cancer tissue.

K Hatake·K Motoyoshi·T Hanamura·N Mukaida·T Kasahara·Y Miura·M Saito
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Abstract

We have found that medium conditioned by a colony-stimulating factor producing tumor derived from a granulocytosis case with lung cancer contained a factor to differentiate a human promyelocytic leukemic cell line (HL-60) to macrophage-like cells that were butyrate esterase-positive and had phagocytosing activity and membrane Fc receptors. This differentiation-inducing factor was not active for a human myeloblastic cell line (KG-1), and was separated from a colony-stimulating factor by its molecular weight and isoelectric point. The conditioned medium did not contain a detectable amount of gamma interferon when tested by bioassay as well as by radioimmunoassay. This is the first report that a human lung cancer tissue produces not only a colony-stimulating factor, but also a differentiation-inducing factor. The conditioned medium is considered to be a good source of differentiation-inducing factor.

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AnimalsCell DifferentiationCulture MediaGranulocytesHumansLeukemia, Myeloid, AcuteLeukocytosisLung NeoplasmsLymphokinesMaleMiceMice, Inbred BALB CNeoplasm TransplantationTumor Cells, CulturedTumor Stem Cell AssayJournal ArticleResearch Support, Non-U.S. Gov't

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K Hatake, K Motoyoshi, T Hanamura, N Mukaida, T Kasahara, Y Miura, M Saito. 1987. Differentiation-inducing factor for a human leukemic cell line produced by colony-stimulating factor producing human lung cancer tissue.. https://pubmed.ncbi.nlm.nih.gov/3477467/

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