PubMed · 3481055
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D Annunziato. 1987. Koplik spots and echo 9 virus.. https://pubmed.ncbi.nlm.nih.gov/3481055/
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Polypeptide 2C is essential for picornavirus replication. Although many data on multiple functions of this highly conserved protein are available, the mechanism of RNA binding is still obscure. In this work, protein 2C of echovirus-9 strain Barty was expressed as a histidine-tagged protein in E. coli followed by nondenaturing purification to homogeneity. After incubation of 2C protein with different kinds of RNA fragments, binding was shown in gel retardation assays. Competition experiments revealed that 2C targets linear RNA unspecifically; however, single-stranded linear DNA does not react with this protein. In contrast to poliovirus, protein 2C of echovirus-9 only recognizes RNA with a low content of secondary structures. This may be a first hint of a different binding specificity of 2C in echo- and polioviruses.
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A 30-year-old man had a "flu-like" syndrome followed by clinical and laboratory evidence of myositis localized in both thighs. Serum neutralization test results were compatible with enterovirus echo 9 infection. Severe myoglobinuria dominated the clinical picture. We discuss other possible manifestations of enterovirus echo 9 infections. To our knowledge, this report is the first describing myositis caused by enterovirus echo 9.