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PubMed · 3501294

Structural studies on the murine granulocyte colony-stimulating factor.

R J Simpson·E C Nice·N A Nicola
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Abstract

Granulocyte-colony stimulating factor (G-CSF) is a glycoprotein hemopoietic growth factor which regulates the production of granulocytes and macrophages. Reversed-phase microbore high-performance liquid chromatography was employed to purify a number of tryptic and Staphylococcus aureus V8 proteinase peptides generated from approximately 400 pmol G-CSF purified from medium conditioned by lungs from mice previously injected with endotoxin. N-Terminal amino-acid sequence analyses were performed on the parent polypeptide and on four tryptic peptides and one Staphylococcus aureus V8 protease peptide, yielding 68 unique amino-acid assignments; this corresponds to approximately 38% of the molecule.

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Amino Acid SequenceAnimalsChromatography, High Pressure LiquidColony-Stimulating FactorsGranulocytesHydrolysisMiceMolecular Sequence DataPeptidesTrypsinJournal Article

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R J Simpson, E C Nice, N A Nicola. 1987. Structural studies on the murine granulocyte colony-stimulating factor.. https://doi.org/10.1515/bchm3.1987.368.2.1327

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