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PubMed · 3516015

Computer-assisted morphometry using video-mixed microscopic images and computer graphics.

C A Curcio·K R Sloan
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Abstract

A microcomputer system has been developed for collection and analysis of morphometric data from video images of histological sections. Microscopic fields of view are visualized on a video monitor and are overlaid with computer-generated graphics by means of a video mixer. Planimetric data are entered by drawing on a digitizing tablet while observing the drawn image superimposed on the tissue. Programs are available to calculate various geometric parameters, count objects in a field, perform descriptive statistics, and measure volume and surface area of a solid from a series of cross sections. A program for point-counting stereology presents single points for scoring within a user-defined reference area. The potential applications of interactive graphics in morphometry are discussed.

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Cell NucleusComputersCytological TechniquesHistological TechniquesSoftwareJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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C A Curcio, K R Sloan. 1986. Computer-assisted morphometry using video-mixed microscopic images and computer graphics.. https://doi.org/10.1002/ar.1092140313

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