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PubMed · 3603039

A defined medium for a fastidious Spiroplasma.

K J Hackett·A S Ginsberg·S Rottem·R B Henegar·R F Whitcomb
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Abstract

A defined medium (H-1) was developed for cultivation of the suckling mouse cataract agent, Spiroplasma mirum, a fastidious member of the class Mollicutes that causes cataracts and chronic brain infection in inoculated neonate mice. The H-1 medium was used to show the importance of sphingomyelin as a growth factor for the culture of the spiroplasma in vitro. The growth of Spiroplasma mirum and the pathology it induces in sphingomyelin-rich tissues in vivo may be related to this dependency.

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AnimalsCataractCulture MediaMiceSpiroplasmaJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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K J Hackett, A S Ginsberg, S Rottem, R B Henegar, R F Whitcomb. 1987-07-31. A defined medium for a fastidious Spiroplasma.. https://doi.org/10.1126/science.3603039

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