Mu insertion duplicates a 5 base pair sequence at the host inserted site.

B Allet

doi:10.1016/0092-8674(79)90193-4

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Mu insertion duplicates a 5 base pair sequence at the host inserted site.

B Allet

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Abstract

Nucleotide sequences were analyzed across the two ends of lysogenic Mu DNA. These ends were cloned separately in lambdapMu hybrid particles that derived from a single Mu lysogen in the lac Z part of lambdaplac5. The obtained data imply that Mu lysogenization was associated with the duplication of 5 base pairs present in lac DNA at the Mu insertion site. As a result of this duplication, Mu DNA is flanked by two copies of five identical base pairs oriented as direct repeats. A similar conclusion has been obtained independently by other investigators with the use of a different Mu lysogen (D. Kamp and R. Kahmann, personal communication). Thus Mu insertion seems to have a striking similarity to typical IS-mediated insertions that were found to be associated with a short DNA duplication at the target site.

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Base Sequence Coliphages DNA, Bacterial DNA, Viral Escherichia coli Lac Operon Lysogeny Translocation, Genetic Journal Article

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B Allet. 1979. Mu insertion duplicates a 5 base pair sequence at the host inserted site.. https://doi.org/10.1016/0092-8674(79)90193-4

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