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Physalaemin-like immunoreactive peptides from rabbit stomach.

W E Wilson·D J Harvan·C Hamm·L H Lazarus·D G Klapper·H Yajima·Y Hayashi
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Abstract

Two physalaemin (PHY)-like immunoreactive peptides, designated PHLIPs, have been purified from extracts of rabbit stomach tissue. Fast atom bombardment/mass spectrometry (FAB/MS) indicated that the m/z values for the PHLIP protonated molecular ions were 867.419 and 796.4. FAB/tandem MS spectra, coupled with a knowledge of the amino acid composition and the aid of a computerized fragment-matching program, indicated the amino acid sequences to be: (formula; see text) The sequences of PHLIPs-7 and -8 were confirmed with synthetic peptides. The PHY-antiserum cross-reactivity of the PHLIPs reflects homology at amino acid residues 1, 3, 4 and 5 for the mammalian and amphibian residues.

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Amino Acid SequenceAmino AcidsAnimalsKininsMass SpectrometryPeptide FragmentsPeptidesPhysalaeminRabbitsRadioimmunoassayStomachJournal Article

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W E Wilson, D J Harvan, C Hamm, L H Lazarus, D G Klapper, H Yajima, Y Hayashi. 1986. Physalaemin-like immunoreactive peptides from rabbit stomach.. https://doi.org/10.1111/j.1399-3011.1986.tb03229.x

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