Studies on polydnavirus transmission.

Abstract

Polydnaviruses are thought to replicate only in the ovaries of certain hymenopteran species. Nevertheless, in the present study, polydnaviral DNA was found to exist in males of the braconid parasitoid species Cotesia melanoscela and in both male and female non-ovarian tissue of an ichneumonid, Hyposoter fugitivus; preliminary results suggest that viral DNA may be present in an unintegrated form, but whether or not it is encapsidated is unknown. Using interstrain genetic crosses, we demonstrated that C. melanoscela males can apparently transmit at least some viral DNA to female progeny. We suggest that polydnavirus DNAs may be present in most if not all tissues of certain parasitoid species, and are probably maintained within parasitoid populations by vertical transmission through the germ line. In parallel experiments, manually injected eggs of the ichneumonid parasitoid (H. fugitivus) survived and hatched in Malacosoma americanum larvae in the apparent absence of exogenous polydnavirus; female parasitoids reared in this manner nevertheless carried virus in their ovaries. Experiments utilizing different strains of C. melanoscela also suggest that per os transmission of polydnaviruses (to parasitoid larvae) does not occur, despite the fact that inoculum viral DNA can be shown to persist for several days in the tissues of parasitized host larvae.