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PubMed · 3916329

Multipoint identification. Experience with an established technique.

L Solaro
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Abstract

The introduction and use of a multipoint inoculation identification scheme in a diagnostic laboratory processing approximately 60,000 specimens per years is described.

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Bacteriological TechniquesCulture MediaGram-Negative BacteriaHumansMicrobial Sensitivity TestsJournal Article

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L Solaro. Multipoint identification. Experience with an established technique.. https://pubmed.ncbi.nlm.nih.gov/3916329/

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Genetically modified cells of Pseudomonas fluorescens, chromosomally marked with genes for bioluminescence, were inoculated into sterile soil microcosms. During incubation for 90 days, viable cell concentration did not change significantly but light output, measured by luminometry, decreased, indicating reduced metabolic activity due to lack of substrates. Amendment with nutrients resulted in parallel increases in both luminescence and dehydrogenase activity. Luminometry therefore enables rapid monitoring of the activity of populations of luminescence-marked microbial inocula in the soil, with greater sensitivity and selectivity than traditional techniques.

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