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Proteome analysis provides insights into sex differences in Holothuria Scabra.

Chuhang Cheng·FeiFei Wu·Yizhi Xu·Chunhua Ren·Ting Chen·Shella Li·Peihong Shen·Fajun Jiang
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Abstract

Sex-determining mechanism is still ambiguous for sea cucumber Holothuria scabra which only manifests gonochorism in gonad. In this study, proteomic analysis was employed to delineate sex-related proteins and genes in gonads of H. scabra, subsequently validated through Quantitative real-time polymerase chain reaction (qRT-PCR). A total of 5,313 proteins were identified via proteome sequencing. Among these, 817 proteins exhibited expression in both the ovary and testis, with 445 proteins displaying up-regulation and 372 proteins showing down-regulation (ovary vs testis). Furthermore, 136 and 69 proteins were identified as ovary-specific and testis-specific Differentially Abundant Proteins (DAPs), respectively. And 9 DAP coding genes which play crucial role in ovary and testis were verified by qRT-PCR. Notably, 24 ovary-bias proteins enriched in ribosome pathway strongly indicated the crucial role of ribosome in ovary. This study serves to furnish novel evidence pertaining to sex differences in H. scabra.

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AnimalsMaleFemaleHolothuriaProteomeOvaryTestisProteomicsSex CharacteristicsJournal ArticleResearch Support, Non-U.S. Gov't

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Chuhang Cheng, FeiFei Wu, Yizhi Xu, Chunhua Ren, Ting Chen, Shella Li, Peihong Shen, Fajun Jiang. 2024-08-29. Proteome analysis provides insights into sex differences in Holothuria Scabra.. https://doi.org/10.1371/journal.pone.0301884

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