PubMed Health⌕ Search

PubMed · 3929369

[A syllectrometric method to study erythrocyte aggregation].

Abstract

The authors show a new method to study erythrocyte aggregation; the results of in vitro study, obtained by modification of several parameters (hematocrit, immunoglobulins, fibrinogen concentrations), are exposed. The authors did not find in vivo correlations between MAE and hemorheological parameters in different diseases; they found low MAE values only in myelomatous subjects. Adding erythrocytes from healthy donors to myelomatous plasma and vice versa, they found low MAE values only in samples containing erythrocytes and plasma from myeloma. These results suggest that many factors, not only erythrocyte aggregability, contribute to determine MAE (Mean Aggregation Entity).

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

D Pieragalli, S Forconi, M Guerrini, A Acciavatti, C Galigani, C Del Bigo, T Di Perri. 1985. [A syllectrometric method to study erythrocyte aggregation].. https://pubmed.ncbi.nlm.nih.gov/3929369/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Blood fluidity is related to the ability to oxidize lipids at exercise.

We previously reported in rugbywomen correlations between RBC deformability and the ability to oxidize at exercise more lipids. This surprising finding might of course be spurious, or reflect the importance of the balance of substrates at exercise on baseline parameters that regulate blood rheology. Actually, the capacity of skeletal muscle to utilize either lipid or carbohydrate as fuels strongly influences whole body metabolism both at rest and during exercise. While the healthy skeletal muscle has substantial metabolic flexibility and is able to switch from predominantly lipid o oxidation during fasting or endurance exercise to increased glucose oxidation in conditions of insulin stimulation, obese individuals and those with type 2 diabetes manifest higher lipid oxidation during insulin-stimulated conditions despite lower rates of lipid oxidation during fasting or prolonged exercise. A low ability to oxidize and to periodically deplete triglyceride in muscle is associated with raised blood lipids. In addition, high carbohydrate oxidation rates in the mitochondrion are likely to promote more free radical generation. An increase in either blood lipids or free radicals is likely to induce profound hemorheological effects. We present here hemorheological studies in various populations with the use of exercise calorimetry in order to assess this switch of substrates. These studies further evidence negative correlations between the ability to oxidize lipids at exercise and parameters of blood viscosity. Correlations found between RBC deformability and the ability to oxidize at exercise more lipids may be due to effects of endurance training on lipid oxidation which may in turn modify both lipid metabolism and free radical generation, thus influencing RBC rheology.

Erythrocyte Aggregation↗

Ultrasound scattering from concentrated suspensions of aggregated red cells in shear flow.

Ultrasound scattering technique is used to investigate dynamics of reversible fractal aggregates in dense suspensions and analyze shear break-up processes of Rayleigh fractal clusters. On the basis of an homogenous fractal flocculation and the hybrid scattering model in the Rayleigh scattering regime, a first order expression of the ultrasound scattering cross-sectional area per unit of volume (backscattering coefficient) is derived for a dense distribution of correlated Rayleigh fractal clusters. From the scaling laws for shear break-up of reversible aggregates in concentrated suspensions, a rheo-acoustical study is then proposed to describe the shear stress dependence of the low frequency scattered power per unit of volume. In a second part, experimental flow dependent changes of the ultrasound backscattering coefficient in a plane-plane flow geometry were reported to analyze shear break-up processes of hardened or deformable red cell aggregates in polymer solution (neutral dextran polymer). Rheo-acoustical experiments were examined within the framework of the effective mean field approximation and the proposed rheo-acoustical model. The ability of ultrasound scattering to determine the critical disaggregation shear stress inducing a complete disaggregation and to give quantitative information on particle surface adhesive energy are finally analyzed.

Erythrocyte Aggregation↗