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UDP-glucuronosyltransferase: fluorometric determination and microcomputer method for bisubstrate kinetics analysis.

J Snegaroff·P Jamet
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Abstract

A method for analysing UDP-glucuronosyltransferase activity in rainbow trout hepatic microsomes is described, using 1-naphthol as a substrate and fluorometric determination of glucuronide. Kinetic constants are computed with a classical plot in a weighted regression. The computer uses the least-squares method for each value of a variable which is set and incremented. To get confidence intervals, the computer generates random values around experimental data (in a confidence interval they determine), and then computes again. With each simulation, a weighted regression with classical secondary plots gives simulated kinetic parameters. From each population of simulated values, an interval containing a given percentage of the population is determined.

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BibTeXRIS

J Snegaroff, P Jamet. 1985. UDP-glucuronosyltransferase: fluorometric determination and microcomputer method for bisubstrate kinetics analysis.. https://doi.org/10.1016/0003-2697(85)90624-4

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