Selective decrease of type I collagen synthesis in Fraser mice skin.

P Kern; L Robert; Y Courtois; M Laurent

doi:10.1016/0167-4781(85)90003-x

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Selective decrease of type I collagen synthesis in Fraser mice skin.

P Kern·L Robert·Y Courtois·M Laurent

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Abstract

Quantification and biosynthesis of type I and type III collagens were determined in skin of control and Fraser mice (CatFraser mutation), which exhibit a genetically determined cataract. Skin organ cultures were labelled with [3H]proline. Pepsin-solubilized collagens were studied using three different approaches: (a) differential salt precipitation at neutral pH, followed by SDS-polyacrylamide gel electrophoresis; (b) differential salt precipitation at acid pH followed by SDS-polyacrylamide gel electrophoresis. (c) CNBr peptide analysis. These methods gave consistent and reproducible results, indicating a selective decrease of type I collagen in Fraser mouse skin as compared to control mouse skin. Metabolic labelling of skin organ cultures showed a decreased specific radioactivity of hydroxy[3H]proline in type I collagen of Fraser mouse skin. The concordant results of these experiments suggest a genetically determined alteration of interstitial collagen metabolism in the Fraser mutation apparently specifically concerning the expression of type I collagen gene(s).

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Animals Collagen Electrophoresis, Polyacrylamide Gel Kinetics Mice Mice, Mutant Strains Mutation Organ Culture Techniques Pepsin A Skin Journal Article Research Support, Non-U.S. Gov't

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P Kern, L Robert, Y Courtois, M Laurent. 1985-12-18. Selective decrease of type I collagen synthesis in Fraser mice skin.. https://doi.org/10.1016/0167-4781(85)90003-x

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