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PubMed · 39606390

DNA-guided CRISPR/Cas12 for RNA targeting.

Abstract

CRISPR-Cas nucleases are transforming genome editing, RNA editing, and diagnostics but have been limited to RNA-guided systems. We present ΨDNA, a DNA-based guide for Cas12 enzymes, engineered for specific and efficient RNA targeting. ΨDNA mimics a crRNA but with a reverse orientation, enabling stable Cas12-RNA assembly and activating trans-cleavage without RNA components. ΨDNAs are effective in sensing short and long RNAs and demonstrated 100% accuracy for detecting HCV RNA in clinical samples. We discovered that ΨDNAs can guide certain Cas12 enzymes for RNA targeting in cells, enhancing mRNA degradation via ribosome stalling and enabling multiplex knockdown of multiple RNA transcripts. This study establishes ΨDNA as a robust alternative to RNA guides, augmenting the potential of CRISPR-Cas12 for diagnostic applications and targeted RNA modulation in cellular environments.

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BibTeXRIS

Carlos Orosco, Santosh R Rananaware, Boyu Huang, Michael P Hanna, M Reza Ahmadimashhadi, Jordan G Lewis, Michael P Baugh, August P Bodin, Sarah J Flannery, Ian H Lange, Zoe R Fang, Vedant N Karalkar, Katelyn S Meister, Piyush K Jain. 2024-11-22. DNA-guided CRISPR/Cas12 for RNA targeting.. https://doi.org/10.1101/2024.11.21.24317744

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