PubMed Health⌕ Search

PubMed · 3992936

[Microbiological research on processed cheese].

Abstract

Microbiologic studies were carried out with a total of 136 batches of 8 assortments of softened cheese to establish the amount and composition of the microbial content of this product. It was found that the total count of mesophilic organisms of 72.8 per cent of the investigated batches reached up to 200 000/g. The content of hygiene-indicative organisms (coliforms and enterococci) was limited: 97 per cent of batches had a coli titer of 1 and over 1. The content of enterococci in 54.4 per cent of the investigated was below 100/g, and in 4.4 per cent it was over 100 000/g. No Salmonella bacteria and plasmacoagulase positive staphylococci in 25, resp., 10 g of the product were found. Contamination with sporeforming aerobic and anaerobic organisms in 72.1, resp., 91.9 per cent reached up to 1000, resp., 100 per gram of the product. The amount of proteolytic bacteria in 61.8 per cent of the batches was up to 1000/g, and in 8 per cent only--up to 100 000/g. The content of yeasts and moulds in 58.8, resp. 83.1 per cent of the batches was below 100/g.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

V Aleksieva, N Kostova, I Mladenova. 1985. [Microbiological research on processed cheese].. https://pubmed.ncbi.nlm.nih.gov/3992936/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Collateral sensitivity-harnessing microbial vulnerabilities as a solution to antimicrobial resistance.

Bacteria exhibit an evolutionary trade-off through their development of collateral sensitivity (CS) which allows them to resist one antibiotic while becoming more vulnerable to another. This vulnerability offers a compelling therapeutic opportunity by selecting against resistant isolates. Laboratory evolution studies, genome sequencing, deep mutagenesis and use of artificial intelligence and machine learning can design the bespoke strategy against multi-drug-resistant bacteria. This review discusses about recent studies that are rationally designed to harness this evolutionary trade-off for the development of alternative antimicrobial strategies. The translational barriers to the clinical implementation of CS are addressed and evidence-based design principles for optimization of CS-guided therapy are discussed.

Bacteria↗

How the social lives of bacteria affect their pangenome.

Although the study of microbes started with type strains and reference genomes, advances in sequencing technology and new interest in mixed microbial communities have made us aware that a single genome cannot and does not reflect the diversity of a given bacterial species. Bacteria rarely occupy an environmental or host niche alone and quickly diversify into strains upon colonization of a new niche. The genetic diversity present within a phylogenetically related set of bacterial strains (the 'pangenome') is influenced by the niche that they occupy and how they interact with the other microorganisms that they share that niche with. In this review, I examine how the social lives of bacteria can affect their genetic diversity and the bioinformatic techniques that we use to detect that diversity.

Bacteria↗

DURABLE: A Workflow for Determining Corrosion-Driving and Protective Microbial Mechanisms.

Microbiologically influenced corrosion (MIC) threatens global infrastructure, causing billions of dollars in annual losses. Its persistence stems from unresolved mechanisms─particularly the metabolites produced by microorganisms that drive or inhibit corrosion─and the microbial community structures. Progress has been hindered by the absence of systematic workflows to rapidly and accurately identify MIC-relevant microorganisms and their functions. Here, we present DURABLE (Detection of Unique Corrosion Resistant or Accelerating Biologics in a Laboratory Environment), a pipeline that couples high-throughput microbial screening with genomic and metabolic workflows. We applied the DURABLE workflow to six diesel tank samples and revealed fuel-dependent microbial community structures, which showed greater diversity and evenness in bacterial communities than their fungal counterparts. The workflow used carbon steel beads to rapidly screen over 80 bacterial isolates for corrosive activity, reducing assay time to approximately 2 days compared with the conventional 30-day metal coupon test. More than 40 isolates were identified as corrosive. Further testing using mass spectrometry analysis revealed corrosion-associated metabolites, which were further validated using electrochemical assays. Thus, DURABLE achieved a ∼15-fold increase in screening speed and provided a scalable and mechanistic framework for dissecting MIC dynamics. We expect this advance will enable the development of precision mitigation strategies in hydrocarbon fuel infrastructure.

Bacteria↗