PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 4073611

[Microanalysis on dry reactive supports].

P Metais·J M Lessinger
View original source ↗Export metadata ↓

Abstract

Among actual technological developments in clinical chemistry, the use of dry reagents fixed on a solid support is particularly advantageous for assays in emergency. Reflectometry is used for the measurement and was analysed in terms of principles and measurement devices. To day, only one apparatus Seralyser is furnished by Ames and we have evaluated its performances. However, other apparatus are in evaluating. Thus, Ektachem Kodak 400, 700 or DT 60 employs multilayer slides. Reflotron from Boehringer allows the direct use of blood samples. Stratus with a fluorimetrie measurement is adapted to drug assays. This new technology some permits interesting applications derived from basic research but arises some problems for the realisation of the as well as the role of the clinical chemist.

Explore related subjects

Blood Chemical AnalysisChemistry, ClinicalColorimetryIndicators and ReagentsKineticsMicrochemistryPhysical PhenomenaPhysicsSpectrophotometryTime FactorsEnglish AbstractJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

P Metais, J M Lessinger. 1985. [Microanalysis on dry reactive supports].. https://pubmed.ncbi.nlm.nih.gov/4073611/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Detection of Delta9-tetrahydrocannabinolic acid A in human urine and blood serum by LC-MS/MS.

Delta9-tetrahydrocannabinolic acid A (Delta9-THCA-A) is the precursor of Delta9-tetrahydrocannabinol (Delta9-THC) in hemp plants. During smoking, the non-psychoactive Delta9-THCA-A is converted to Delta9-THC, the main psychoactive component of marihuana and hashish. Although the decarboxylation of Delta9-THCA-A to Delta9-THC was assumed to be complete--which means that no Delta9-THCA-A should be detectable in urine and blood serum of cannabis consumers--we found Delta9-THCA-A in the urine and blood serum samples collected from police controls of drivers suspected for driving under the influence of drugs (DUID). For LC-MS/MS analysis, urine and blood serum samples were prepared by solid-phase extraction. Analysis was performed with a phenylhexyl column using gradient elution with acetonitrile. For detection of Delta9-THCA-A, the mass spectrometer (MS) (SCIEX API 365 triple-quadrupole MS with TurboIonSpray source) was operated in the multiple reaction monitoring (MRM) mode using the following transitions: m/z357 --> 313, m/z357 --> 245 and m/z357 --> 191. Delta9-THCA-A could be detected in the urine and blood serum samples of several cannabis consumers in concentrations of up to 10.8 ng/ml in urine and 14.8 ng/ml in serum. The concentration of Delta9-THCA-A was below the Delta9-THC concentration in most serum samples, resulting in molar ratios of Delta9-THCA-A/Delta9-THC of approximately 5.0-18.6%. Only in one case, where a short elapsed time between the last intake and blood sampling is assumed, the molar ratio was 18.6% in the serum. This indicates differences in elimination kinetics, which need to be investigated in detail.

Blood Chemical Analysis↗