Differential light scattering from spherical mammalian cells.

A Brunsting; P F Mullaney

doi:10.1016/s0006-3495(74)85925-4

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Differential light scattering from spherical mammalian cells.

A Brunsting·P F Mullaney

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Abstract

The differential scattered light intensity patterns of spherical mammalian cells were measured with a new photometer which uses high-speed film as the light detector. The scattering objects, interphase and mitotic Chinese hamster ovary cells and HeLa cells, were modeled as (a) a coated sphere, accounting for nucleus and cytoplasm, and (b) a homogeneous sphere when no cellular nucleus was present. The refractive indices and size distribution of the cells were measured for an accurate comparison of the theoretical model with the light-scattering measurements. The light scattered beyond the forward direction is found to contain information about internal cellular morphology, provided the size distribution of the cells is not too broad.

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Cell Division Cell Line Cell Nucleus Culture Media Cytoplasm Female HeLa Cells Humans Light Mathematics Mitosis Models, Biological Ovary Photography Scattering, Radiation Serum Albumin, Bovine Staining and Labeling Time Factors Journal Article

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A Brunsting, P F Mullaney. 1974. Differential light scattering from spherical mammalian cells.. https://doi.org/10.1016/s0006-3495(74)85925-4

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