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Quantification of uromucoid: a simplified method.

I Akesson·H Haugen·E Enger
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Abstract

As a replacement of current time consuming techniques, a radial immunodiffusion method which is suitable for routine determination of uromucoid in urine in described. The method is based on denaturation of uromucoid by sodium dodecyl sulphate at 37 degrees C before application on the immunoplates. A diffusion period of 30 h at 37 degrees C was chosen. The determination is uninfluenced by variations of urinary electrolytes, freezing and thawing, and storage at 4 degrees C for 3 weeks or at -20 degrees C for several months. The method can also be applied to lyophilized urines.

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Electrophoresis, Polyacrylamide GelHumansImmunodiffusionMethodsMucoproteinsSodium Dodecyl SulfateTime FactorsJournal Article

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BibTeXRIS

I Akesson, H Haugen, E Enger. 1978. Quantification of uromucoid: a simplified method.. https://doi.org/10.3109/00365517809108409

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