PubMed · 4246773
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B Brisou. 1970. [Black-beetle control].. https://pubmed.ncbi.nlm.nih.gov/4246773/
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We present a genome assembly from a male specimen of Neocrepidodera transversa (flea beetle; Arthropoda; Insecta; Coleoptera; Chrysomelidae). The genome sequence has a total length of 671.30 megabases. Most of the assembly (93.58%) is scaffolded into 21 chromosomal pseudomolecules, including the X chromosome. The mitochondrial genome has also been assembled and is 17.02 kilobases in length. Gene annotation of this assembly on Ensembl identified 13,840 protein-coding genes.
Firefly luciferase, containing an average of seven free sulfhydryls per two 50 000-dalton polypeptides, was modified by various sulfhydryl reagents. The differential reactivities of the sulfhydryls in luciferase protected by substrates allow one to define three categories of these groups: Class SH-III contains three sulfhydryls that are not involved in enzymatic activity. Class SH-II contains two sulfhydryls whose modification by different reagents causes varying effects on activity ranging from 0 to 60% inactivation. These sulfhydryls are not essential but may be important structurally or sterically. Class SH-I contains two sulfhydryls that are protected by substrates, either dehydroluciferyl adenylate or dehydroluciferin alone, and are located at or near the active site. The SH-I sulfhydryls are vicinal in the enzyme as demonstrated by their ability to form a disulfide bond. They have also been shown to exist on a single polypeptide chain. Modification of the SH-I groups by most reagents results in complete loss of enzymatic activity; reaction with methyl methanethiosulfonate produces an enzyme that emits only red light whereas native luciferase emits yellow-green light. Evidence is presented that the modified enzyme, while catalytically active, has a distorted active site. It is concluded that these two SH-I sulfhydryls are not essential for activity.
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