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Clinical investigation of serum deoxyribonuclease: I. Analysis of serum deoxyribonuclease activity in comparison with normal and after endoscopic retrograde pancreatography.

A Funakoshi·T Kimura·H Wakasugi·H Ibayashi
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Abstract

Serum Deoxyribonuclease (DNase) micro-assay method was developed using 32P-labelled E. coli DNA as substrate. The serum DNase showed maximum activity at pH 7.5. It required Mg+ for activity, and was inhibited by EDTA or EGTA. The enzyme was also inhibited by actin (60-65%) or bovine pancreatic DNase I antibody (40-45%). The serum DNase activity was markedly increased following endoscopic retrograde pancreatography (ERP) examination. These results imply that serum DNase activity is mostly at least 60-65% pancreatic DNase I.

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ActinsAmylasesAntibodiesDeoxyribonucleasesEdetic AcidEgtazic AcidEndoscopyHumansHydrogen-Ion ConcentrationMagnesiumMethodsPancreasPancreatitisRadiographyComparative StudyJournal Article

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A Funakoshi, T Kimura, H Wakasugi, H Ibayashi. 1979. Clinical investigation of serum deoxyribonuclease: I. Analysis of serum deoxyribonuclease activity in comparison with normal and after endoscopic retrograde pancreatography.. https://doi.org/10.1007/bf02773730

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