Phosphorus determination in phosphoglycerides from thin-layer chromatograms.

A F Rosenthal; S C Han

Home/Archive/PubMed

PubMed · 4305714

Phosphorus determination in phosphoglycerides from thin-layer chromatograms.

A F Rosenthal·S C Han

View original source ↗Export metadata ↓

Abstract

Phosphoglyceride phosphorus is readily determined directly on silica gel removed from thin-layer chromatograms, without elution, by a nondigestive procedure with a sulfuric-periodic acid reagent. The method is specific for hydrolyzable phosphoglyceride containing two acid-hydrolyzable groups. Cariolipin reacts only partially, while sphingomyelin, diether lecithin, and phosphonate cephalin fail completely to react.

Explore related subjects

Chromatography, Thin Layer Glycerophosphates Indicators and Reagents Lysophosphatidylcholines Methods Periodic Acid Phosphatidylcholines Phospholipids Phosphorus Sphingomyelins Sulfuric Acids Journal Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

A F Rosenthal, S C Han. 1969. Phosphorus determination in phosphoglycerides from thin-layer chromatograms.. https://pubmed.ncbi.nlm.nih.gov/4305714/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Monolithic porous polymer layer for the separation of peptides and proteins using thin-layer chromatography coupled with MALDI-TOF-MS.

Plates for thin-layer chromatography (TLC) with an attached layer of porous polymer monolith have been prepared and used for the separation of small molecules, peptides, and proteins. The 50-200-mum. thin poly(butyl methacrylate-co-ethylene dimethacrylate) layers were prepared in situ using UV-initiated polymerization. Precise control of the reaction conditions enables the preparation of monolithic layers with a well-defined porous structure that determines the chromatographic performance. Compared to conventional TLC and high-performance TLC using precoated layers based on silica, the small layer thickness and absence of any binder is expected to improve both retention characteristics and separation efficiency of the polymer-based monolithic thin-layer chromatographic plates. Spots of the separated compounds were first detected using typical UV imaging. Since the monolithic thin layers can be also prepared directly on the stainless steel MALDI carrier plate, the separation in TLC format can be coupled with MALDI-TOF-MS. Application of a conventional MALDI matrix facilitated desorption and ionization of peptides and proteins for molecular weight determination of the separated compounds.

Chromatography, Thin Layer↗

PubMed2007

Separation of solanesol in tobacco leaves extract by slow rotary counter-current chromatography using a novel non-aqueous two-phase solvent system.

Non-aqueous solvent system composed of sunflower oil-ethanol was found to be suitable for the separation of lower polarity components. Employing this solvent system, 3 g of tobacco leaves extract containing 15% solanesol was separated using a slow rotary counter-current chromatograph equipped with an 1100 mL column made of 5.7 mm I.D. convoluted PTFE tube. The separation yielded 1.5 g of yellow oily product in which solanesol occupied 26.8% determined by HPLC. The tobacco tar and other main impurities in the tobacco leaves extract were removed after slow rotary counter-current chromatographic separation. The separation processing may be developed as a technique for producing a solanesol product with food grade.

Chromatography, Thin Layer↗

PubMed2006

Selective thin-layer chromatography of 4-R-1,2,4-triazoles.

A convenient and selective TLC of 4-R-1,2,4-triazoles (Rtrz) and nitrogen containing aromatic rings is presented (molecules 1-11). This simple revelation method implies a complexation of heterocycles to Co(II) ions on a TLC plate, followed by a subsequent modification of the reduction potential which enables oxidation of Co(II) by permanganate.

Chromatography, Thin Layer↗