[Fluorescent probe study of staphylococcal cell wall charges].

S V Tuchin; V I Panasenko; V V Ignatov; N N Larchenko

PubMed · 43463

[Fluorescent probe study of staphylococcal cell wall charges].

S V Tuchin·V I Panasenko·V V Ignatov·N N Larchenko

Abstract

The effect of pH and MgCl2 concentration on the fluorescence of 1-anilinonaphthalene-8-sulfonate (ANS), an anion probe, was studied in suspensions of Staphylococcus aureus. The maximum of ANS fluorescence shifted towards the short wavelength region of the spectrum and its intensity increased when the cells were incubated in the medium with low pH and high ionic strength. The results are discussed in terms of electrostatic interactions between the anions of the probe and the negative space charge of the cell wall.

Explore related subjects

Anilino Naphthalenesulfonates Cell Wall Chlorides Electron Probe Microanalysis Hydrogen-Ion Concentration Magnesium Osmolar Concentration Spectrometry, Fluorescence Staphylococcus aureus English Abstract Journal Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

S V Tuchin, V I Panasenko, V V Ignatov, N N Larchenko. [Fluorescent probe study of staphylococcal cell wall charges].. https://pubmed.ncbi.nlm.nih.gov/43463/

Cite the original work for its findings. Save a collection to share your selection of sources.

[Fluorescent probe study of staphylococcal cell wall charges].

Abstract

Explore related subjects

Keep this discovery

Related citations

The binding of 8-anilinonaphthalene-1-sulphonate to a fixed concentration of unenergised and succinate-energised submitochondrial particles.

Reaction of purified (Na,K)-ATPase with the fluorescent sulfhydryl probe 2-(4'-maleimidylanilino)naphthalene 6-sulfonic acid. Characterization and the effects of ligands.

Spectral analysis of fluorescence of 8-anilino-1-naphthalenesulfonate in chemoreception with a white plasmodium of Physarum polycephalum. Evidence for conformational change in chemoreceptive membrane.