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PubMed · 4423793

Numerical method for equilibrium fluorescence polarization.

D Mavis·H C Schapiro·W B Dandliker
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D Mavis, H C Schapiro, W B Dandliker. 1974. Numerical method for equilibrium fluorescence polarization.. https://doi.org/10.1016/0003-2697(74)90421-7

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The present paper describes a theoretical study on the mechanism underlying the reaction of cellular glutathione (GSH) with polyunsaturated fatty acid ozonides. The reaction can be catalyzed by glutathione S-transferases and leads to detoxication of the ozonides. Semi-empirical molecular orbital computer calculations suggest that the reaction of glutathione with ozonides involves a nucleophilic attack at one of the carbon atoms of the ozonide ring, instead of at one of the peroxidic oxygen atoms of the ozonide ring. This implies a mechanism different from that of the glutathione S-transferase-mediated reaction with hydroperoxides, previously proposed for the glutathione-dependent detoxication of fatty acid ozonides.

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