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PubMed · 4562479

Fermentation process for double-stranded ribonucleic acid, an interferon inducer.

B D Lago·J Birnbaum·A L Demain
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Abstract

Double-stranded ribonucleic acid (ds-RNA) isolated from Escherichia coli infected with bacteriophage MS2 is a potent interferon inducer. High levels of ds-RNA are formed in nonpermissive cells infected with MU9, an amber coat protein mutant of MS2. This mutant has been used to develop a process for large-scale ds-RNA production. Preparation of quantities of MU9 lysate sufficient for ds-RNA production in fermentors is described. Over 300 mug of ds-RNA/ml can be accumulated after MU9 infection of cultures grown to high density in corn steep liquor medium. This is approximately 300 times the amount of ds-RNA made by MS2 infection of cells grown in tryptone medium. Maximum ds-RNA formation requires only 3 hr. The ds-RNA is stable and remains inside nonaerated cells for at least 17 hr.

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AirColiphagesCulture MediaEscherichia coliFermentationInterferon InducersLysogenyMethodsMutationPeptonesRNA, ViralTemperatureTrypsinViral ProteinsVirus ReplicationZea maysJournal Article

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B D Lago, J Birnbaum, A L Demain. 1972. Fermentation process for double-stranded ribonucleic acid, an interferon inducer.. https://doi.org/10.1128/am.24.3.430-436.1972

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