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Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells.

D L Robertson·N L Baenziger·D C Dobbertin·R E Thach
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Abstract

The RNA-dependent DNA polymerase present in intracisternal A-type particles from mouse myeloma tumor cells has been studied. This polymerase can use either endogenous A particle RNA or an exogenous synthetic polynucleotide [poly (rA)] as a template. The DNA reaction product is small (4S-10S) and over 90% of it hybridizes to A particle RNA, whereas up to 50% of it hybridizes to murine sarcoma-leukemia virus RNAs. The RNA isolated from purified A particles is generally of low molecular weight (5S-15S) but contains small amount of 70S and 35S components. These results suggest that A-type particles may be related to C-type oncornaviruses.

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AnimalsCell FractionationCell LineCentrifugation, Density GradientDNA, ViralDactinomycinGammaretrovirusMiceMice, Inbred BALB CMicroscopy, ElectronMolecular WeightNucleic Acid HybridizationPlasmacytomaPolyethylene GlycolsPolynucleotidesRNA, ViralRNA-Directed DNA PolymeraseTemplates, GeneticThymine NucleotidesTritiumJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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D L Robertson, N L Baenziger, D C Dobbertin, R E Thach. 1975. Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells.. https://doi.org/10.1128/jvi.15.2.407-415.1975

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