PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 4629043

Characteristics of lambda p4, a lambda derivative containing 9 per cent excess DNA.

J Weil·R Cunningham·R Martin·E Mitchell·B Bolling
View original source ↗Export metadata ↓

Abstract

The source did not provide an abstract. Follow the original record for more information.

Explore related subjects

Centrifugation, Density GradientColiphagesCrosses, GeneticDNA, ViralEdetic AcidEscherichia coliGenetics, MicrobialHot TemperatureLysogenyMorphogenesisMutationRecombination, GeneticViral Plaque AssayVirus ReplicationJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

J Weil, R Cunningham, R Martin, E Mitchell, B Bolling. 1972. Characteristics of lambda p4, a lambda derivative containing 9 per cent excess DNA.. https://doi.org/10.1016/0042-6822(72)90388-1

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1992

Insemination of HIV-negative women with processed semen of HIV-positive partners.

Many HIV-discordant couples want to have children so much that they are willing to abandon condom-protected sexual intercourse irrespective of the risks. Previous testing in our laboratory showed that gradient centrifugation followed by a swim-up procedure effectively removed HIV-1-infected cells from the semen of HIV-seropositive men. 85 HIV-discordant couples were screened for fertility; 29 women were found suitable for a timed insemination course with the processed semen of their HIV-seropositive partner. None of the inseminated women seroconverted, and 17 pregnancies were achieved in 15 women. All 10 babies born to these mothers remain HIV seronegative. The findings may help in the counselling of such couples and also give them hope of having healthy babies.

Centrifugation, Density Gradient↗
PubMed1992

Purification and properties of Saccharomyces cerevisiae RNA polymerase II general initiation factor a.

RNA polymerase II initiation factor a was purified to apparent homogeneity from yeast whole cell extract and consisted of two highly charged polypeptides with apparent masses of 66 and 43 kDa. Separation and renaturation of the subunits showed that both were required for transcription activity. The native mass of factor a was estimated to be 240-260 kDa by gel filtration, but its sedimentation rate in a glycerol gradient was similar to that of a much smaller globular protein, suggesting an extended conformation. Factor a was required for utilization of six different eukaryotic promoters in vitro, indicating a general role in promoter-directed transcription by yeast RNA polymerase II.

Centrifugation, Density Gradient↗