PubMed · 4754722
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R P Novick, K Smith, R J Sheehy, E Murphy. 1973-10-15. A catenated intermediate in plasmid replication.. https://doi.org/10.1016/0006-291x(73)91150-9
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As part of an ongoing laboratory survey process improvement program, we evaluated the addition of water to liquid scintillation cocktail to improve wipe test counting efficiency. Both polar and non-polar 3H and 14C-labeled compounds were used as model contaminants. Our results support the recommendations in the literature regarding the addition of water to scintillation cocktail We found an increase in the counting efficiency of the water-soluble material as a function of water content of the cocktail, but also observed a decrease in the efficiency of detection of the non-polar compound. The offsetting effects are believed to be the result of increased solubility of the polar compounds in water and increased quench of the already solubilized non-polar compound. The finding that adding water to the cocktail brought counting efficiencies of both polar and non-polar molecules to roughly the same value is novel and allows the use of a single quench curve for each radionuclide, regardless of chemical form.
Edema, proteinuria, hypertension (EPH) gestosis is accompanied by an increase of collagen content and premature replacement of hyaluronic acid by sulfated glycosaminoglycans both in the umbilical cord arteries and in Wharton's jelly. The effect of EPH gestosis on elastin content and metabolism in the umbilical cord arterial wall was the aim of this work. Studies were performed on normal umbilical cord arteries and those taken from newborns of mothers with EPH gestosis. Elastin was isolated from the arterial wall and quantified by a dye-binding method. Biosynthesis and degradation of this protein was evaluated by a pulse-chase experiment with the use of 14C-proline. It was found that EPH gestosis is associated with a significant reduction of elastin content in the umbilical cord arteries as a result of decrease in elastin biosynthesis rate and accelerated degradation of this protein. The replacement of elastin by collagen, and hyaluronate by sulfated glycosaminoglycans, may decrease the hydration of arterial wall and reduce its elasticity. Such rearrangement of extracellular matrix of the umbilical cord arteries may affect mechanical properties of these vessels and disturb fetal blood circulation.
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