PubMed HealthSearch

PubMed · 4877661

Automatic sample collector for continuous cultures.

Abstract

An automatic sample collector is described that samples growing cultures sequentially. It can be programmed to collect at preselected intervals (usually several hours apart) for a prescribed period (usually from minutes to hours). Samples are collected serially but during intermediate time periods; when the sample is not required, it is disposed of by diverting it into a discard reservoir. This device collects culture samples on a predetermined schedule and stores them under refrigeration for study at a later time.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

W J Eisler. 1968. Automatic sample collector for continuous cultures.. https://doi.org/10.1128/am.16.9.1381-1382.1968

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

Oligonucleotide labeling methods. 3. Direct labeling of oligonucleotides employing a novel, non-nucleosidic, 2-aminobutyl-1,3-propanediol backbone.

Novel CE-phosphoramidite (7a-e) and CPG (8a, c, d, e) reagents have been prepared from a unique 2-aminobutyl-1,3-propanediol backbone. The reagents have been used to directly label oligonucleotides with fluorescein, acridine, and biotin via automated DNA synthesis. The versatile 2-aminobutyl-1,3-propanediol backbone allows for labeling at any position (5', internal, and 3') during solid phase oligonucleotide synthesis. Multiple labels can be achieved by repetitive coupling cycles. Furthermore, the 3-carbon atom internucleotide phosphate distance is retained when inserted internally. Using this method, individual oligonucleotides possessing two and three different reporter molecules have been prepared.

Automation

[Routine application of HLA class II oligotyping. Value of automatization].

HLA class II typing by DNA amplification and hybridization with allele-specific oligonucleotides has led to an improvement of the overall typing accuracy. In an attempt to apply this procedure to a large-scale analysis, we sought to implement an automation system using the Biomek 1,000 robotic work-station. This system allows a rapid preparation of many consecutive samples and avoids the risk of human errors. It is now routinely used in several laboratories.

Automation