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Biomedical subjects

W J Eisler

Publications and source records attributed to W J Eisler.

At least 19 recordsLinked to original sources
PubMed1988

Multisample parallel array fraction collector: application in DNA alkaline elution studies.

A fraction collector designed specifically to be compatible with the analytical techniques of alkaline and neutral elution of DNA from carcinogen-treated cells is described. This apparatus, which is operated by microchip controls, permits simultaneous collection of as many as 20 eluted samples directly into standard- or small-size scintillation vials contained in manufacturer's shipping cartons. This instrument saves operator time, offers considerable flexibility, and has several fail-safe features.

Chemical Fractionation↗
PubMed1976

Improved temperature control of the Technicon tissue processor paraffin bath.

Modifications of the Auto-Technicon Tissue Processor Paraffin Bath are described. The modification are a combination of electrical and mechanical changes that will ensure long-term reliability of the unit and dependable temperature maintenance of the paraffin. Extensive testing has verified that modified units are able to stabilize temperature within a range of 0.25C over many months of continuous operation.

Histological Techniques↗
PubMed1972

High-intensity vapor lamp for biological research.

A vapor arc light source has been adapted to the study of the lethal action on bacteria of near-ultraviolet (UV) and visible light. Its use makes possible much shorter exposure times than could be obtained from previously available sources. The output of radiant energy is sufficient to provide a fairly detailed action spectrum for lethality in the long-UV and visible region without the addition of exogenous sensitizers. Populations of cells of Escherichia coli WP2 were inactivated through five log(10) cycles with light at 460 nm. Significant inactivation also was obtained with light at 550 and 650 nm.

Bacteria↗
PubMed1970

Automated continuous culture of mammalian cells in suspension.

A system has been developed for the continuous culture of mammalian cells in suspension. The system maintains constant cell concentrations (monitored as the degree of light scattering) over a wide range of previously selected values by automatic additions of known amounts of medium and simultaneous withdrawals of equal volumes of cell suspension.

Animals↗
PubMed1968

Electronically controlled continuous culture device.

A photocell-controlled continuous culture device, a Nephelostat, is described that maintains a wide variety of cultures of microorganisms in balanced growth. This Nephelostat controls concentrations of bacteria within +/-3% over a cell concentration range of 10(6) to 10(9) cells per ml. Growth rates are recorded so that changes in the growth rate are observed over small increments of time. Spontaneous and caffeine-induced mutation rates of two strains of Escherichia coli were compared under Nephelostat and chemostat conditions.

Automation↗
PubMed1968

Automatic sample collector for continuous cultures.

An automatic sample collector is described that samples growing cultures sequentially. It can be programmed to collect at preselected intervals (usually several hours apart) for a prescribed period (usually from minutes to hours). Samples are collected serially but during intermediate time periods; when the sample is not required, it is disposed of by diverting it into a discard reservoir. This device collects culture samples on a predetermined schedule and stores them under refrigeration for study at a later time.

Automation↗