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The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.

J A Manni·H J Müller-Eberhard
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Abstract

A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma(1)-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.

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AnimalsBlood Protein ElectrophoresisChromatographyChromatography, GelComplement System ProteinsHemolysisHumansImmunochemistryImmunoelectrophoresisMethodsRabbitsUltracentrifugationJournal Article

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J A Manni, H J Müller-Eberhard. 1969-11-01. The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.. https://doi.org/10.1084/jem.130.5.1145

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