PubMed · 5527622
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A Robertson. 1970. Some optimum problems in individual selection.. https://doi.org/10.1016/0040-5809(70)90045-6
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Cis-regulatory elements (CREs) are the genetic DNA fragments bound by transcription factors (TFs). CREs function as molecular switches that precisely modulate the dosage and spatiotemporal patterns of gene expression. The systematic identification of CREs not only facilitates the annotation of the functional non-coding genome but also provides essential insights into the architecture of gene regulatory networks and sheds light on an accurate selection of the target sites for genetic engineering of crops. In this review, we summarize the current high-throughput methodologies used for identifying CREs, illustrate the associations between CREs and agronomic traits in horticultural crops, and discuss how CREs can be exploited to facilitate crop breeding.
Genetic variability of melon seed proteins was studied by separation of seed protein by isoelectric focusing in immobilized pH gradient (IEF-IPG) under denaturing conditions. A routine procedure was developed for IEF-IPG of hundreds of individual melon seeds per day. A group of 74 accessions from 19 morphologically distinct groups and from different geographic origin were studied by IEF-IPG using pH gradients of 4-10, 4-7 and 6-10. The electrophoretic analysis of the 74 accessions showed 270 reproducible seed protein bands of which 70 were variable. Genetic evaluation led to the conclusion that at least 20 loci govern the variation found. The phylogenetic trees constructed using the protein data on one hand and the morphological data on the other hand were compared and their use was evaluated. A number of commercial Cantaloup F1 hybrid descendants derived from the F1 hybrids by diplohaploidization or single plot descent were studied by IEF-IPG using pH gradients of 4-7 and 6-10. Among the F1 hybrids and their descendants 265 reproducible protein bands could be identified of which 72 were variable as to presence versus absence. The genetic interpretation of the protein pattern as found by IEF-IPG and the use of IEF-IPG in plant breeding was discussed. It was concluded that IEP-IPG of melon seed proteins is a valuable tool in breeding.
A population of ten morphologically similar inbred lines of pepper (Capsicum annuum L.) has been investigated for polymorphism of seed proteins by two-dimensional (2-D) electrophoresis with immobilized pH gradients. To reveal as much variation as possible, both the water- and the urea/detergent-soluble protein fraction were electrophoretically analyzed and subsequently visualized by silver staining. The 2-D patterns were subjected to computer analysis to be able to establish genetic variation. A high number of the seed proteins were found to be variable as to presence/absence: these were 68 out of 184 reproducible water-soluble proteins and 34 out of 419 reproducible urea/detergent-soluble proteins. Comparison of the 2-D data of the water-soluble and the urea/detergent-soluble proteins, which represent the biggest part of all extractable seed proteins, showed that both protein fractions have proteins in common, but the variable proteins found in both fractions were non-identical. The difference of variability scored in both solubility fractions was discussed. Genetic distances between all pairs of inbred pepper lines were calculated and a genetic tree was constructed. A correlation analysis was carried out to correct for genetic linkage and for secondary modifications, to have a more proper estimate of genetic distances. In both cases the dendrograms showed two distinct genetic groups of five inbred lines. This electrophoretic study was done in order to utilize the genetic distance data in breeding for heterosis. The genetic distance data presented will be used to validate the assumption that there is a higher chance to achieve better hybrid performance when the genetic distance between the parents is as great as possible.(ABSTRACT TRUNCATED AT 250 WORDS)