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Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis.

E E Sercarz·F Modabber
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Abstract

A sensitive method for detecting cells containing antibody to beta-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.

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AnimalsAntigen-Antibody ReactionsFluoresceinsFluorometryGalactosidasesGlycosidesImmunoassayMicePhotometrySpleenJournal Article

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E E Sercarz, F Modabber. 1968-02-23. Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis.. https://doi.org/10.1126/science.159.3817.884

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