PubMed · 6079079
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S Katagiri, S Ozasa. 1967. X-ray microanalysis attachment for electron microscope.. https://pubmed.ncbi.nlm.nih.gov/6079079/
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The current major limitation in protein micro-structural and -functional studies is our inability to purify and manipulate microgram or smaller quantities of material. Both high performance liquid chromatography (HPLC) and polyacrylamide gel electrophoresis (PAGE) have been widely used in attempts to address such needs. This review summarizes the current status of preparative and analytical applications for both methods, and suggests which directions the micro-separations field will take within the next few years.
A procedure, based on the complex formation of malachite green with phosphomolybdate under acidic conditions, to measure inorganic orthophosphate in the nanomolar range is described. The addition of polyvinyl alcohol is required to stabilize the dye-phosphomolybdate complex. The advantages of the assay are simplicity, stability of the reagents, and high sensitivity. Due to the high permissible acidity in the assay (0.9 N H2SO4), the method can be adapted easily to measure nanomolar amounts of phosphate, liberated from organic compounds like phosphoproteins and phospholipids after wet digestion.