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PubMed · 6085125

[Rapid detection method for macroamylasemia].

H Oda·E Furusaka·Y Sasagawa·K Nishii·R Awatsu·K Kamisaka·H Maezawa
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AmylasesChromatography, High Pressure LiquidHumansMacromolecular SubstancesEnglish AbstractJournal Article

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H Oda, E Furusaka, Y Sasagawa, K Nishii, R Awatsu, K Kamisaka, H Maezawa. 1984. [Rapid detection method for macroamylasemia].. https://pubmed.ncbi.nlm.nih.gov/6085125/

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Effects of calyculin A on amylase release in streptolysin-O permeabilized acinar cells.

The effects of the phosphatase inhibitors calyculin A and okadaic acid on amylase release from streptolysin-O permeabilized rat pancreatic acini were investigated. Both agents induced similar biphasic effects with moderate potentiation of calcium-stimulated amylase release at medium and strong inhibition at higher concentrations. Calyculin A was thirty times more potent than okadaic acid and at 100 nM totally inhibited calcium-induced amylase release while 3 microM okadaic acid reduced amylase release by 78%. 100nM calyculin A also completely inhibited GTP gamma S-potentiated amylase release and partially inhibited phorbol ester potentiated secretion. The data indicate that inhibition of a serine/threonine phosphatase, probably a type 1 phosphatase, leads to inhibition of calcium-induced amylase release in permeabilized pancreatic acini.

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