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PubMed · 6099315

Creating new restriction sites by silent changes in coding sequences.

J W Little·D W Mount
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Abstract

We present methods for identifying a useful type of DNA site--one that can be mutated to create a new restriction site within a coding region without changing the amino acid sequence. These "latent sites" are abundant--silent mutations creating one of 44 different 6-bp or 8-bp recognition sites were found at relatively high density, roughly one latent site per 9 bp, in the eleven genes tested. Our analysis suggests that site-directed mutagenesis can be used to refashion coding sequences at will for flexible analysis.

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Base SequenceCodonComputersDNA Restriction EnzymesGenesGenetic CodeMethodsMutationSubstrate SpecificityComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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J W Little, D W Mount. 1984. Creating new restriction sites by silent changes in coding sequences.. https://doi.org/10.1016/0378-1119(84)90033-7

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