A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

B Wasylyk; P Chambon

doi:10.1093/nar/9.8.1813

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A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

B Wasylyk·P Chambon

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Abstract

We have previously shown that a T to G transversion at the second T of the conalbumin "TATA" box drastically decreases specific initiation of transcription by RNA polymerase B (1). We now report that a T to A transcription at the same nucleotide also drastically decreases transcription, suggesting that these mutations influence transcription mainly by altering nucleotide recognition rather than helix stability. Small deletions of 2, 3 and 4 nucleotides in the "TATA" box also drastically decrease specific initiation of transcription.

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Animals Base Composition Base Sequence Conalbumin DNA Restriction Enzymes DNA, Recombinant Egg Proteins Mutation Plasmids RNA Polymerase II Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't

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B Wasylyk, P Chambon. 1981-04-24. A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.. https://doi.org/10.1093/nar%2F9.8.1813

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