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PubMed · 6272687

Further studies on complementation between mutants of Clostridium perfringens.

T Tatsuki·T Imagawa·H Kitajima·Y Higashi·M Chazono·Y Yanagase·T Amano
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Abstract

1. Mutants devoid of lambda- and kappa-toxin and hemagglutinin (HA), respectively, were isolated from Cl. perfringens PB6K. The lambda- and HA- mutants could be classified into a and b groups by complementation but the kappa- mutants were all of the a group. 2. All b group mutants isolated, irrespective of the marker used for isolation, were pleiotropically negative or leaky with respect to theta-, lambda- and kappa-toxin and HA production. 3. Lambda-toxin produced by complementation was proved to be a rennet-like protease. 4. The activities of 12 extracellular enzymes, including sialidase, of several b group strains and the parent PB6K were compared, but no definite differences were observed. From this finding, the productions of these enzymes were concluded not to be regulated by the same mechanism as theta-, lambda- and kappa-toxin and HA. 5. Cl. perfringens CN3870 was also studied. Findings were similar to those on PB6K except for very low activity of HA.

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Bacterial ToxinsClostridium perfringensEnzymesGenes, BacterialGenetic Complementation TestGenetic MarkersHemagglutininsMutationJournal Article

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T Tatsuki, T Imagawa, H Kitajima, Y Higashi, M Chazono, Y Yanagase, T Amano. 1981. Further studies on complementation between mutants of Clostridium perfringens.. https://pubmed.ncbi.nlm.nih.gov/6272687/

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