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PubMed · 6284844

[Surface ultrastructural study on the human endometrial interstitium using digestion method (author's transl)].

H Suzuki·S Nakayama·M Oshima·Y Yoshida
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Abstract

A scanning electron microscopic study on the human endometrium, using Evan's method and modifications, revealed the following findings. a) 8N . HCl-Collagenase method: High-speed digestion of the specimen made the determination of the adequate digestion time difficult, due to conc. HCl and high processing temperature. b) 1N . HCl-Collagenase method: Long digestion time produced many artifacts as shrinkage of the cells or formation of small holes on them. c) Tripsin-Collagenase method: Tissue breaking often resulted before the adequate digestion. d) Hyaluronidase-Collagenase method: This is most satisfactory in classifying glands and vessels, and digestion time is the shortest of all methods.

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EndometriumFemaleHumansMicrobial CollagenaseMicroscopy, Electron, ScanningTrypsinEnglish AbstractJournal Article

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H Suzuki, S Nakayama, M Oshima, Y Yoshida. 1982. [Surface ultrastructural study on the human endometrial interstitium using digestion method (author's transl)].. https://pubmed.ncbi.nlm.nih.gov/6284844/

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