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5'-Nucleotidase activity in subjects with abnormal lymphocyte function.

P S Gerber·H G Herrod
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Abstract

5'-Nucleotidase (5'N) activity was determined in lymphocytes from patients with immunodeficiency diseases and in T-lymphocyte subpopulations from normal subjects. Cells from two patients with DiGeorge syndrome, with normal levels of B cells, and one patient with partial DiGeorge syndrome were found to have diminished 5'N activity. Utilizing monoclonal antibodies to deplete T-lymphocyte subpopulations, we found similar levels of 5'N in the cells remaining after depletion of OKT4- or OKT8-positive cells when 5'N values were determined after overnight incubation. If 5'N activity was determined on the day of the fractionation, however, OKT4-depleted cells had approximately threefold greater enzyme activity. These studies indicate that 5'N activity may vary in T lymphocyte subpopulations depending upon cell culture conditions. Diminished levels of 5'N activity are seen in patients with abnormal T-lymphocyte differentiation, as well as abnormalities of B-lymphocyte differentiation.

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5'-NucleotidaseAdultAgammaglobulinemiaAntibodies, MonoclonalDiGeorge SyndromeFemaleHumansImmunologic Deficiency SyndromesInfantMaleNucleotidasesT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P S Gerber, H G Herrod. 1983. 5'-Nucleotidase activity in subjects with abnormal lymphocyte function.. https://doi.org/10.1007/bf00915346

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