Rat mitochondrial DNA polymorphism: sequence analysis of a hypervariable site for insertions/deletions.

G G Brown; L J DesRosiers

doi:10.1093/nar/11.19.6699

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Rat mitochondrial DNA polymorphism: sequence analysis of a hypervariable site for insertions/deletions.

G G Brown·L J DesRosiers

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Abstract

Three closely related variants of rat (Rattus norvegicus) mtDNA have been shown to differ in the number of T residues found in a run of Ts (light strand) which spans the junction between the tRNACys and tRNATyr genes. The number of Ts in the repeat varies from 6 to 8 in these DNAs. Another, less closely related, R. norvegicus variant has a run of 5 Ts at this site and in the related species, Rattus rattus, a run of 4 Ts is found. In R. norvegicus mtDNA runs of 5 As and 5 Gs are found just to the 3' side of the variable T repeat, and it is suggested that the three runs of repeated nucleotides may stabilize heteroduplexes which result from strand slippage and which give rise to the insertions and/or deletions. Among 17 mtDNA clones derived from an individual with the 8T repeat, one clone was found which possessed a 9T repeat. This variant may represent an additional DNA type originally present within the individual.

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Animals Base Sequence Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements DNA, Mitochondrial Genetic Variation Kidney Mitochondria Mitochondria, Liver Polymorphism, Genetic Rats Species Specificity Comparative Study Journal Article Research Support, Non-U.S. Gov't

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G G Brown, L J DesRosiers. 1983-10-11. Rat mitochondrial DNA polymorphism: sequence analysis of a hypervariable site for insertions/deletions.. https://doi.org/10.1093/nar%2F11.19.6699

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